Advisor

Ma, Din-Pow

Committee Member

Jenkins, Johnie N.

Committee Member

Saha, Sukumar

Committee Member

Peng, Zhaohua

Committee Member

Li, Jiaxu

Date of Degree

1-1-2013

Document Type

Graduate Thesis - Open Access

Major

Biochemistry

Degree Name

Master of Science

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

The ubiquitin-proteasome proteolysis pathway is responsible for the degradation of abnormal and short-lived proteins to regulate many important biochemical activities in eukaryotes. By employing affymetrix microarray analysis, we have identified a novel ubiquitin ligase E3 gene GhRING2 that is expressed differentially between two G. hirsutum lines - Texas Marker-1 and Chromosome Substitution Line CS-B25. The complete GhRING2 gene sequence was obtained by genomic and cDNA walking. The expression of GhRING2 in cotton fiber is developmentally regulated, suggesting that the ubiquitin-proteasome pathway may regulate cotton fiber growth and development. Using a yeast two-hybrid assay GhRING2 was found to interact with a PROTODERMAL FACTOR1 (GhPDF1) protein. GhPDF1 is expressed preferentially in immature ovules and fiber initials and the gene has been suggested to play a role in cell fate determination and fiber development. Pull down and plasmid swap assays were employed to confirm this interaction.

URI

https://hdl.handle.net/11668/20311

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