Theses and Dissertations

Issuing Body

Mississippi State University


Huston, Carla

Committee Member

Hostetler, Doug

Committee Member

Zhang, Shuping

Date of Degree


Document Type

Graduate Thesis - Open Access


Veterinary Science

Degree Name

Master of Science


College of Veterinary Medicine


Department of Pathobiology and Population Medicine


The purpose of this study was to characterize Johne’s disease in Mississippi cattle. Nine hundred eighteen animals from 23 sale barns in Mississippi were tested for Mycobacterium avium subspecies paratuberculosis (MAP). Ten milliliters of blood and 4-10 grams of feces were collected from cattle over two years of age presented to the attending auction veterinarian. Information obtained at the time of collection included the animal’s sex, type, and reproductive status. Serum samples were screened by an enzyme-linked immunosorbent assay (ELISA) for MAP. Shedding status was determined using polymerase chain reaction (PCR) on corresponding fecal samples. Overall, 17.3% (4/23) of sale barns had at least one animal sero-positive for MAP and 0.54% (5/918) were PCR positive. These results show a Johne’s disease prevalence similar to the estimate of 0.4% of animals infected found by the USDA NAHMS Beef ‘97 study, emphasizing the need for continued prevention and control practices.