Mississippi State University
Date of Degree
Graduate Thesis - Open Access
Agricultural Life Sciences (Biochemistry)
Master of Science
College of Agriculture and Life Sciences
Department of Biochemistry and Molecular Biology
Ethanol fermentation of highly concentrated aflatoxin-contaminated corn (Zea mays) was conducted on a lab scale to determine if aflatoxin concentrated in the distilled ethanol and/or dry distillers grain end-products. Alliquots of fermented mash, distilled ethanol, stillage, and dry distillers grain (DDG) were analyzed via LC-MS/MS and immunoassay detection methods for aflatoxin. Results indicate that aflatoxin does not greatly concentrate during fermentation in the DDGs and is undetectable in distilled ethanol. Addition of binders, MTB-100®, to aflatoxin-contaminated DDGs showed great reduction in aflatoxin concentrations when analyzed via LC-MS/MS. Also, an experiment investigating detoxification of aflatoxin using Clorox® was conducted. Results obtained from LC-MS/MS showed a positive correlation of decreased aflatoxin levels with increasing Clorox® levels following a logarithmic trend.
Singleterry, Rebecca Burgett, "Comparing detection methods of aflatoxin and exploring aflatoxin decontamination methods" (2010). Theses and Dissertations. 1103.