Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Willard, Scott

Committee Member

Schmidt, Ty

Date of Degree

12-10-2010

Document Type

Graduate Thesis - Open Access

Major

Agricultural Life Sciences (Biochemistry)

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry and Molecular Biology

Abstract

Ethanol fermentation of highly concentrated aflatoxin-contaminated corn (Zea mays) was conducted on a lab scale to determine if aflatoxin concentrated in the distilled ethanol and/or dry distillers grain end-products. Alliquots of fermented mash, distilled ethanol, stillage, and dry distillers grain (DDG) were analyzed via LC-MS/MS and immunoassay detection methods for aflatoxin. Results indicate that aflatoxin does not greatly concentrate during fermentation in the DDGs and is undetectable in distilled ethanol. Addition of binders, MTB-100®, to aflatoxin-contaminated DDGs showed great reduction in aflatoxin concentrations when analyzed via LC-MS/MS. Also, an experiment investigating detoxification of aflatoxin using Clorox® was conducted. Results obtained from LC-MS/MS showed a positive correlation of decreased aflatoxin levels with increasing Clorox® levels following a logarithmic trend.

URI

https://hdl.handle.net/11668/15582

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