Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Ryan, Peter L.

Committee Member

Feugang, Jean M. N.

Committee Member

Christiansen, David L.

Date of Degree

1-1-2012

Document Type

Graduate Thesis - Open Access

Major

Agriculture

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Animal and Dairy Sciences

Abstract

This study was carried out primarily to evaluate the cryo-tolerance of equine semen from four stallions through assessing the spermatozoa motion characteristics with Computer-Assisted Sperm Analysis (CASA). Four stallions were collected during the breeding season (summer). For each ejaculate, fresh and cryopreserved samples were taken for sperm motility characteristics evaluation. Data analysis demonstrated that sperm cells of stallions were significantly affected by (P<0.05) cryodamage. Stallion (A) was cryotolerant, and was classified as a good freezer, whereas stallion (D) was not and classified as a poor freezer regardless of the concentration of sperm. In addition, a concentration of 0.4 x 109 sperm cells/ml had higher percentages of rapid sperm and velocity parameters (P<0.05) compared to 0.8 x 109 sperm/ml. Further research is necessary to identify potential biomarkers for good and poor freezer stallions.

URI

https://hdl.handle.net/11668/19419

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