Author

Keshav Sharma

Advisor

Klink, Vincent

Committee Member

Lawrence, Gary W.

Committee Member

Donaldson, Janet R.

Date of Degree

1-1-2015

Document Type

Graduate Thesis - Open Access

Major

Biological Sciences

Degree Name

Master of Science

College

College of Arts and Sciences

Department

Department of Biological Sciences

Abstract

Soybean cyst nematode (SCN), a major pathogen of soybean worldwide, causes huge losses in soybean production. Various approaches including cloning of genes to combat this devastating disease help to better understand the cellular function and immune responses of plants. Membrane fusion genes are the important regulatory parts of vesicular transport system, which works through packaging of intracellular compounds and delivering them to apoplast or nematode feeding sites to induce an incompatible reaction. The incompatible nature of membrane fusion proteins such as SNAP25, Munc18, Syntaxin, Synaptobrevin, NSF, Synaptotagmin and alpha-SNAP are conserved in eukaryotes and regulate the intracellular function to combat abiotic and biotic stress in plants. Overexpression of these genes in G. max [Williams 82(PI518671)] which is a susceptible cultivar of soybean to nematodes resulted in a reduction of the SCN population providing further insights of molecular and genetic approaches to solve the SCN problems in agriculture.

URI

https://hdl.handle.net/11668/20055

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