Date of Degree
Graduate Thesis - Open Access
Storage of canine packed red blood cells (pRBCs) can increase erythrocyte phosphatidylserine (PS) expression and eicosanoid concentrations. The objective of this study was to determine the effects of leukoreduction on erythrocyte PS expression and eicosanoid concentrations in stored units of canine pRBCs. Units of whole blood were leukoreduced (LR) or non-leukoreduced (non-LR), and stored (10 and 21 days) as pRBCs. Samples were collected at donation, and before and after a simulated transfusion. PS expression was measured by flow cytometry, and concentrations of arachidonic acid (AA), prostaglandin F2α (PGF2α), prostaglandin E2 (PGE2), prostaglandin D2 (PGD2), thromboxane B2 (TXB2), 6-keto-prostaglandin F1α (6-keto-PGF1α) and leukotriene B4 (LTB4) were quantified by liquid chromatography-mass spectrometry. Our study demonstrated that PS expression on the surface of erythrocytes was not affected by leukoreduction or storage duration. Additionally, the passage of blood through a leukoreduction filter causes an immediate and dramatic increase in TXB2 and PGF2α concentrations, but these concentrations then decrease during subsequent storage. Despite leukoreduction, the concentration of 6-keto-PGF1α continued to increase during storage and simulated transfusion. Overall, when compared to non-LR units, the addition of a leukoreduction step prior to storage had a minimal impact on the accumulation of eicosanoids in canine units of pRBCs. While leukoreduction may be beneficial in other aspects of transfusion medicine, based on the results in this study, using leukoreduction to decrease PS expression and eicosanoid concentrations does not appear to be effective.
Muro, Samantha, "Effects of Leukoreduction and Storage on Erythrocyte Phosphatidylserine Expression and Eicosanoid Concentrations in Units of Canine Packed Red Blood Cells" (2017). Theses and Dissertations MSU. 1763.