Mississippi State University
Elder, Steven H.
Simpson, Chartrisa LaShan
Eells, Jeffrey B.
Date of Degree
Graduate Thesis - Open Access
Master of Science (M.S.)
James Worth Bagley College of Engineering
Department of Agricultural and Biological Engineering
Female sheep exposed to excess testosterone (T) in utero display symptoms similar to those observed in women with polycystic ovarian syndrome (PCOS). Prenatal T-treated ewes display masculinized sexual behavior and increased food-reward seeking behavior. A neural substrate critical for these goal-directed behaviors is the dopaminergic system in the ventral tegmental area. We have recently shown that in adult ewes dopamine expression in the VTA is increased by prenatal T exposure. In this thesis, I tested the hypothesis that alterations of the VTA dopamine system by prenatal-T are caused via activation of androgen (AR) and/or insulin receptors (IR). Analysis of immunohistochemical staining shows an increase of tyrosine hydroxylase (TH) expression, AR, or IR, along with changes in co-expression of AR/TH and IR/TH. These changes were blocked or reversed by prenatal treatments with flutamide or rosiglitazone, or postnatal treatments with rosiglitazone or metformin, implicating AR and IR in alterations of the VTA
Steadman, Casey Jean, "Effects of Prenatal Testosterone on Ventral Tegmental Area Dopamine Neurons in Sheep Model for Polycystic Ovarian Syndrome" (2014). Theses and Dissertations. 1781.