Theses and Dissertations

Issuing Body

Mississippi State University


Nicodemus, Molly C.

Committee Member

Memili, Erdogan

Committee Member

Hopper, Richard M.

Date of Degree


Document Type

Graduate Thesis - Open Access


Animal Science

Degree Name

Master of Science (M.S.)


College of Agriculture and Life Sciences


Department of Animal and Dairy Sciences


Current equine sperm cryopreservation methods fail to reliably prevent damages to important cellular structures such as the cell membrane and DNA. The objective of this study was to determine the effects of supplementing a stallion semen extender with 1 or 10 mM resveratrol on post-thaw sperm characteristics. Results showed that sperm death was increased with 10 mM compared to both the control and 1 mM (P < 0.05). DNA fragmentation was increased in the 1 mM treatment compared to the control (P < 0.05). ROS activity was reduced the most in the 10 mM with differences between all groups (P < 0.05). Membrane integrity was not different between groups (P > 0.05). Motility of the control was higher than the treatment groups (P < 0.05). Resveratrol was able to reduce ROS but was unable to preserve motility or viability at the concentrations tested.