Theses and Dissertations

Issuing Body

Mississippi State University


Peebles, E. David

Committee Member

Kiess, S. Aaron

Committee Member

Willeford, O. Kenneth

Committee Member

Zhai, Wei

Date of Degree


Document Type

Graduate Thesis - Open Access


Poultry Science

Degree Name

Master of Science


College of Agriculture and Life Sciences


Department of Poultry Science


The Musculus complexus (pipping muscle) plays a primary role in the hatching of the chick from the eggshell at the end of its embryonic life. Various metabolic and cellular changes have been associated with the pipping muscle during development. These studies profiled the pipping muscle at the molecular level by identifying proteins which are associated with the developmental changes. In the first phase of the study, protein expression profile of the Day 13 chicken embryo pipping muscle was obtained using DDF with nano HPLC mass spectrometric analyzer. Identified proteins were categorized based on Gene Ontology. In the second phase, pipping muscle lymph was profiled from Day 20 chicken embryo using PCT with LTQ-Orbitrap mass spectrometric analyzer. The identification of constituent proteins of the piping muscle provides a better understanding of the complex cellular processes and functionality of the pipping muscle with potential benefits for improving hatchability in the poultry industry.