Theses and Dissertations

Author

Jiayuan Jia

Issuing Body

Mississippi State University

Advisor

Lu, Shien

Committee Member

Sabanadzovic, Sead

Committee Member

Peterson, Daniel

Date of Degree

1-1-2016

Document Type

Graduate Thesis - Open Access

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

An aerobic, Gram-negative, rod shaped and polarlagellated bacterium, designated strain MS586, was isolated from soybean rhizosphere in Mississippi. The taxonomic position of MS586 was determined using a polyphasic approach. Analysis of the housekeeping genes supported the novel position of MS586. The results were also supported by average nucleotide identity (ANI) values. Based on these data, it is proposed that strain MS586 represents a novel species, Pseudomonas mississippiensis, within the genus Pseudomonas. The type strain is MS586. Strain MS586 showed a broad-spectrum of antimicrobial activity against plant pathogenic bacteria and fungi that are economically important in agriculture. Preliminary studies using transposon-based mutagenesis showed that the gltB gene was associated with production of antifungal activity against the indicator fungus Geotrichum candidum. The research findings of strain MS586 have provided insights into its potential use as a biocontrol agent in plant disease management.

URI

https://hdl.handle.net/11668/18868

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