Mississippi State University
Date of Degree
Graduate Thesis - Open Access
Plant and Soil Sciences
Master of Science (M.S.)
College of Agriculture and Life Sciences
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology
An aerobic, Gram-negative, rod shaped and polarlagellated bacterium, designated strain MS586, was isolated from soybean rhizosphere in Mississippi. The taxonomic position of MS586 was determined using a polyphasic approach. Analysis of the housekeeping genes supported the novel position of MS586. The results were also supported by average nucleotide identity (ANI) values. Based on these data, it is proposed that strain MS586 represents a novel species, Pseudomonas mississippiensis, within the genus Pseudomonas. The type strain is MS586. Strain MS586 showed a broad-spectrum of antimicrobial activity against plant pathogenic bacteria and fungi that are economically important in agriculture. Preliminary studies using transposon-based mutagenesis showed that the gltB gene was associated with production of antifungal activity against the indicator fungus Geotrichum candidum. The research findings of strain MS586 have provided insights into its potential use as a biocontrol agent in plant disease management.
Jia, Jiayuan, "Identification and Genetic Characterization of Antimicrobial Activities of Pseudomonas Mississippiensis, A Novel Bacterial Species Isolated from Soybean Rhizosphere" (2016). Theses and Dissertations. 2542.