Theses and Dissertations

Issuing Body

Mississippi State University


French, W. Todd

Committee Member

Donaldson, Janet

Committee Member

Brown, Lewis R.

Committee Member

Hernandez, Rafael

Date of Degree


Document Type

Graduate Thesis - Open Access

Degree Name

Master of Science


College of Arts and Sciences


Department of Biological Sciences


Wastewater has been shown to contain the necessary nutritive requirements for the growth of microorganisms. The term, oleaginous, has been given to a classification of microorganisms know to produce up to twenty percent of the weight as oil. This study is designed to examine the potential accumulation of lipids within an oleaginous consortium grown on synthetic wastewater. Potential of the fluorescent stain, Nile red, as a lipid detector is also emphasized. Percentages of extractables greater than thirtyive percent were achieved within the oleaginous consortium using a nitrogen-limited medium. Low pH was found to increase the percentages of extractables. Xylose was shown to be a more optimal carbon source for accumulation than glucose. Nile red was shown to bind to intracellular inclusions and may be useful in monitoring lipid accumulation in industrial settings.



Biodiesel||Nile Red||Yeast||Oleaginous