French, W. Todd
Brown, Lewis R.
Date of Degree
Graduate Thesis - Open Access
Master of Science
College of Arts and Sciences
Department of Biological Sciences
Wastewater has been shown to contain the necessary nutritive requirements for the growth of microorganisms. The term, oleaginous, has been given to a classification of microorganisms know to produce up to twenty percent of the weight as oil. This study is designed to examine the potential accumulation of lipids within an oleaginous consortium grown on synthetic wastewater. Potential of the fluorescent stain, Nile red, as a lipid detector is also emphasized. Percentages of extractables greater than thirtyive percent were achieved within the oleaginous consortium using a nitrogen-limited medium. Low pH was found to increase the percentages of extractables. Xylose was shown to be a more optimal carbon source for accumulation than glucose. Nile red was shown to bind to intracellular inclusions and may be useful in monitoring lipid accumulation in industrial settings.
Ford, Andrew Thompson, "Lipid Accumulation in Synthetic Wastewater-Grown Oleaginous Microorganisms" (2012). Theses and Dissertations MSU. 2959.