Theses and Dissertations

Issuing Body

Mississippi State University


Wardlaw, Jennifer L.

Committee Member

McLaughlin, Ron M., Jr.

Committee Member

Frondoza, Carmelita G.

Date of Degree


Document Type

Graduate Thesis - Open Access


Veterinary Medical Science

Degree Name

Master of Science


College of Veterinary Medicine


Veterinary Medical Science Program


Little research has focused on the involvement of oxidative stress as it relates to the pathophysiology of osteoarthritis (OA); while inflammation has been extensively studied. The present study evaluates the ability to modulate the response of canine chondrocytes to both inflammation and oxidative stress in an in-vitro model. Chondrocytes were incubated and then stimulated to under-go oxidative stress by using hydrogen peroxide or inflammation using interleukin-1 beta and tumor necrosis factor alpha. For inhibition of oxidative stress an antioxidant, N-acetyl-cysteine, was used prior to induction with hydrogen peroxide in a subset of chondrocytes. Measures of oxidative stress were superoxide dismutase and reduced glutathione. Prostaglandin E2 was used as a measurement of inflammation. Chondrocytes responded appropriately to both oxidative stress and inflammation. The antioxidant N-acetyl-cysteine provided adequate protection against oxidative stress. Oxidative stress and inflammation should be considered to play a role in the pathophysiology of canine OA.



canine||chondrocyte||oxidative stress||inflammation||osteoarthritis