Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Sparks, Darrell L., Jr.

Committee Member

Brown, Ashli

Committee Member

Shan, Xueyan

Date of Degree

1-1-2013

Document Type

Graduate Thesis - Open Access

Major

Biochemistry

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

Globalization and the rise of fish importation has led to an increase in mislabeling. To combat this problem, analytical and molecular methods have been employed. First, nitrofuran metabolites were extracted, hydrolyzed, and derivatized in channel catfish, swai, and tilapia. Utilizing high performance liquid chromatography coupled with triple quadrupole mass spectrometry, derivatized metabolites were detected at levels of 1 ng/mL with coefficients of determination greater than 0.998. Recoveries greater than 90% and relative standard deviation less than 17% indicate that the method is successful. Secondly, chip based electrophoresis coupled with restriction fragment length polymorphism was used for the species differentiation. By analyzing restriction digestion products, fragmentation patterns from fin-clip and muscle could consistently differentiate different species requiring two or fewer endonucleases for positive identification. This method of screening reduces the expertise, time, and expense required to reduce fish mislabeling. In tandem, these methodologies could significantly reduce the dangers of fish mislabeling.

URI

https://hdl.handle.net/11668/18513

Comments

antibiotics||fish||fingerprinting||molecular biology||mass spectrometry

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