Novel Spectral and Biochemical Indices for Profiling Animal Stress and Reproductive States
Willard, Scott T.
Date of Degree
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Graduate Thesis - Open Access
Agriculture and Life Sciences
College of Agriculture and Life Sciences
Biological samples (e.g. blood, urine) contain significant amounts of information with regard to physiological states, such as stress and reproduction. The detection and diagnosis of these states often require hormone analysis or specific instrumentation. From a holistic perspective, near infrared (NIR) spectroscopy is used to detect and differentiate physiological states using spectral indices. In a quantitative context, three techniques were compared for their ability to provide accurate and reliable biochemical indices to determine urinary hormone concentrations. The objectives in this study were (1) to map NIR profiles of reproductive metabolites in water, (2) to differentiate estrous, pregnancy, and parturition using NIR, (3) to evaluate urinary normalization methods across stress and reproductive profiles, and (4) to validate an assay for the quantification of stress levels in urine and skin swabs. To investigate these objectives biological samples from mares, giant pandas (Ailuropoda melanoleuca) and Colorado boreal toads (Anaxyrus boreas boreas) were analyzed.
Counsell, Kristen Rae, "Novel Spectral and Biochemical Indices for Profiling Animal Stress and Reproductive States" (2018). Theses and Dissertations MSU. 3318.