Theses and Dissertations

Issuing Body

Mississippi State University


Zappi, Mark E.

Committee Member

French, W. Todd

Committee Member

George, Clifford E.

Committee Member

Toghiani, Hossein

Committee Member

Brown, Lewis R.

Date of Degree


Document Type

Graduate Thesis - Open Access


Chemical Engineering

Degree Name

Master of Science


James Worth Bagley College of Engineering


Dave C. Swalm School of Chemical Engineering


In recent years economic, environmental, and strategic concerns over the use of petroleum resources have led to increased interest in renewable resources. Biomass gasification produces a synthesis gas composed of primarily carbon monoxide, hydrogen, and carbon dioxide, which can be fermented into a variety of fuels and chemicals. This study evaluated the performance of a two-stage approach to batch synthesis gas fermentations. The first stage employs a rich medium optimized for cell growth, while the second stage is designed to maximize production of acetic acid from synthesis gas. This two-stage approach is hypothesized to be more metabolically efficient than previous single-stage designs. This study presents the evaluation of known acetic acid producing organisms described in the literature, and efforts to isolate a novel microbial catalyst for synthesis gas fermentations. Finally, new techniques were developed and implemented in order to develop a more effective system for batch synthesis gas fermentations.



acetic acid||synthesis gas||fermentation||homoacetogen