Author

Xiaojun Wang

Advisor

Memili, Erdogen

Committee Member

Kaya, Abdullah

Committee Member

Karsi, Attila

Committee Member

Peebles, David

Date of Degree

8-1-2010

Document Type

Graduate Thesis - Open Access

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Animal and Dairy Sciences

Abstract

In the field of mammalian reproduction, the success rate of fertilization largely relies on spermatozoa fertility potential. Total proteins change to a large degree during gametogenesis to activate gametes. There are some techniques that are most popular for proteomic studies of fertilization, including SDS-PAGE, 2D-DIGE, Western Blotting and Immunocytochemistry, as well as Mass Spectrometry. Proteins and their cofactors play important roles at different stages of gametogenesis, fertilization and embryo development. However, insufficiencies in the construction mode of fertility hinder the techniques in determination the sperm fertility. This study focused on identifying and tracking crucial proteins for fertility based on comparison between high- and lowertility sperms by means of 2D-DIGE and immunoblotting. We identified 18 proteins that varied significantly, including Outer dense fiber 2 (ODF2) of sperm tails and Manganous superoxide dismutase (MnSOD). Differences in these proteins suggest that posttranslational modification and deoxydation of sperm proteins might be associated with fertility.

URI

https://hdl.handle.net/11668/15328

Comments

fertility||ODF2 and MnSOD||proteins

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