Theses and Dissertations

Author

Yan Li

Issuing Body

Mississippi State University

Advisor

Lawrence, Gary

Committee Member

Klink, Vincent

Committee Member

Balbalian, Clarissa

Committee Member

Lu, Shien

Date of Degree

8-17-2013

Original embargo terms

MSU Only Indefinitely

Document Type

Graduate Thesis - Campus Access Only

Major

Agricultural Sciences

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

Molecular diagnostic assays have been developed and utilized to diagnose and to confirm the diagnoses of many plant-parasitic nematodes. We screened several gene sequences of soybean cyst nematode (SCN) [Heterodera glycines, Ichinohen] for their use as molecular markers. A methodology then was developed to use them to detect and quantify the number of H. glycines directly from Mississippi soil. A novel procedure utilizing multiple databases containing nematode DNA and EST sequences was developed to assist in the selection of SCN primers used in the PCR and qPCR assays. In vitro testing demonstrated that the DNA primers and probes developed from the novel procedure for the qPCR assays could accurately detect the presence of SCN. Subsequent testing resulted in a trend of increasing observed numbers of SCN contributing to increasing estimates by qPCR.

URI

https://hdl.handle.net/11668/19922

Comments

Metagenomic Soybean Cyst Nematode DNA||qPCR conditions||Competence||Hg-unc 78

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