Theses and Dissertations

Issuing Body

Mississippi State University


Allen, Peter J.

Committee Member

Colvin, Michael E.

Committee Member

Mischke, Charles C.

Date of Degree


Document Type

Graduate Thesis - Open Access


Wildlife, Fisheries and Aquaculture

Degree Name

Master of Science


College of Forest Resources


Department of Wildlife, Fisheries and Aquaculture


White Crappie (Pomoxis annularis) and Black Crappie (P. nigromaculatus) are popular North American gamefish; however, frequent fluctuations in year class strength present a management challenge for recreational fisheries. Intensive aquaculture production has the potential to address this challenge through controlled hatchery reproduction for supplemental stocking, but further study is needed to refine and optimize techniques. Therefore, this study evaluated the effects of hormone injection timing on latency period and spawning success, examined effective cryopreservation techniques for black-stripe Black Crappie sperm (a preferred hatchery phenotype), and compared simulated spring duration on out-of-season spawning success. Latency period for White Crappie did not depend on the diel time of gonadotrophin-releasing hormone injection. Cryopreservation of black-stripe Black Crappie sperm and subsequent fertilization of White Crappie eggs was more effective using 5% dimethyl-sulfoxide than 10% methanol. A longer duration at final spring spawning conditions (3 vs. 2 weeks) increased egg fertilization in out-of-season spawning experiments.



Crappie||White Crappie||Black Crappie||latency period||GnRHa||sperm cryopreservation||out-of-season spawning