Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Donaldson, Janet R.

Committee Member

Thornton, Justin

Committee Member

Lawrence, Mark L.

Date of Degree

5-12-2012

Document Type

Graduate Thesis - Open Access

Major

Biological Sciences

Degree Name

Master of Science

College

College of Arts and Sciences

Department

Department of Biological Sciences

Abstract

Listeria monocytogenes is a food-borne pathogen responsible for the disease listeriosis. The infectious process depends upon survival in high bile salt conditions encountered throughout the gastrointestinal tract, including the gallbladder. However, it is not clear how bile salt resistance mechanisms are induced, especially under physiologically relevant conditions. This study sought to determine how L. monocytogenes responds to bile salts under anaerobic conditions. The study found resistance to be strain specific and not dependent upon virulence. Changes in the expressed proteome were analyzed using multidimensional protein identification technology coupled with electrospray ionization tandem mass spectrometry. A general response among virulent and avirulent strains found significant alterations in intensity of cell wall associated proteins, DNA repair proteins, protein folding chaperones and oxidative response proteins. Strain viability was correlated with an initial osmotic stress response followed by strain specific proteins associated with biofilm formation in EGDe and a transmembrane efflux pump in F2365.

URI

https://hdl.handle.net/11668/17915

Comments

proteomic analysis||anaerobic||bile salts||Listeria monocytogenes

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