Theses and Dissertations

Issuing Body

Mississippi State University


Willard, Scott

Committee Member

Kouba, Andrew

Committee Member

Ryan, Peter

Date of Degree


Document Type

Graduate Thesis - Open Access


Animal Physiology

Degree Name

Master of Science


College of Agriculture and Life Sciences


Animal Physiology Program


The aim of this study was to compare the effects of arginine vasotocin (AVT) administration in the endangered boreal toad (Bufo boreas boreas) and common Fowler’s toad (Bufo fowleri). The objectives of this study were to determine if AVT could elicit: 1) calling, and 2) amplexus behaviors. Toads were paired into single male:female groups and administered AVT at varying concentrations: 0.1, 1.0, 5.0, 10.0 and 25.0 μg/g and in different combinations: 1) only male was treated; 2) only female was treated, and 3) both male and female treated. AVT failed to stimulate any breeding behavior in the boreal toad, but the administration of AVT to both B. Fowleri genders significantly affected the duration of amplexus (p<0.0347). Also, the concentration of AVT significantly affected the length of amplexus (p<0.0429) and call frequency (p<0.0294). These results will be valuable for breeding programs where animals are failing to show natural reproductive behavior.



calling||arginine vasotocin||reproduction||amphbian||amplexus||Bufo fowleri||Bufo boreas boreas||hormone