Advisor

Elder, Steven H.

Committee Member

Gilbert, Jerome A.

Committee Member

Bumgardner, Joel D.

Committee Member

McLaughlin, Ronald M.

Date of Degree

1-1-2004

Document Type

Graduate Thesis - Open Access

Degree Name

Master of Science

College

James Worth Bagley College of Engineering

Abstract

Due to mechanical loading, cartilage experiences distortional change, volumetric change, and fluid flow. Research has shown cells to be responsive to unconfined compression, a load that produces all three conditions. To isolate the factor(s) responsible for chondrogenesis, the first goal of this research was to design and implement a device for the application of shear deformation to cells. Secondly, using this device, Stage 23/24 chick limb bud cells were suspended in 2% alginate and subjected to 20% shear deformation at 1 Hz. for two hours daily for three days. Gene expression, DNA content, sGAG content, and cartilage nodule formation were determined after eight days in culture and compared to results obtained for non-loaded cells. Results indicated that shear deformation at the applied level did not have a significant effect on chondrogenesis in Stage 23/24 chick limb bud cells, suggesting that this cell type is not extremely sensitive to distortional change.

URI

https://hdl.handle.net/11668/21263

Comments

chondrogenesis||mechanotransduction||shear deformation||chick limb bud

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