Theses and Dissertations

Issuing Body

Mississippi State University


French, W. Todd

Committee Member

Downer, Donald N.

Committee Member

Hernandez, Rafael

Date of Degree


Document Type

Graduate Thesis - Open Access


Biological Sciences

Degree Name

Master of Science


College of Arts and Sciences


Department of Biological Sciences


Producing biodiesel from triacylglycerol (TAG) generates glycerol as a byproduct which could be recycled and used to grow the oleaginous yeast Rhodotorula glutinis. R. glutinis has the ability to produce up to 70% of its weight in the form of TAG. This study is designed to determine the effects of glycerol on the TAG and fatty acids produced by R. glutinis. After 24 hrs, R. glutinis cultured on medium containing dextrose, xylose, glycerol, dextrose and xylose, xylose and glycerol, or dextrose and glycerol accumulated 16, 12, 25, 10, 21, and 34% TAG on a dry weight basis, respectively. The fatty acids derived from R. glutinis were mostly saturated, however, cells cultivated on glycerol alone had the highest degree of unsaturated fatty acids (53%). Growth on dextrose may be enhanced by the addition of glycerol, but it cannot be determined if using glycerol as a secondary carbon substrate enhances lipid production.



fatty acid||Rhodotorula glutinis||glycerol||triacylglycerol||biodiesel|oleaginous yeats