Theses and Dissertations

Issuing Body

Mississippi State University


Memili, Erdogan

Committee Member

Nicodemus, Molly C.

Committee Member

Jousan, F. Dean

Date of Degree


Original embargo terms

Visible to MSU only for 1 year

Document Type

Graduate Thesis - Open Access


Agriculture (Animal Science)

Degree Name

Master of Science


College of Agriculture and Life Sciences


Department of Animal and Dairy Sciences


The objective of this study was to uncover molecular and cellular dynamics in spermatozoa from Holstein bulls with Good (11 bulls) and Poor (5 bulls) cryotolerance. Post-thaw sperm kinetics, membrane integrity, and DNA fragmentation were assessed. Data was analyzed using principal component analysis. The spermatozoa from Good bulls had a higher number of cells with intact membranes (P = 0.029), nonragmented DNA (P = 0.018), post-thaw viability (P < 0.001) in comparison to those of the Poor bulls. The Good bulls also had a faster average path velocity (VAP; P = 0.017), straight-line velocity (VSL; P = 0.036), a greater distance average path (DAP; P = 0.006) and distance straight line (DSL; P = 0.011). No differences were found in total antioxidant capacity (TAC), number of live cells or other kinetic parameters between spermatozoa from Good and Poor groups, showing that no one characteristic can be used to determine cryotolerance.