Title

Use of the Lowry and Bradford Protein Assays to Measure Bacterial Abundances in a Sandstone Reservoir

Issuing Body

Mississippi State University

Advisor

Lynch, F. Leo

Committee Member

Brown, Lewis R.

Committee Member

Kirkland, Brenda

Date of Degree

1-1-2003

Original embargo terms

MSU Only Indefinitely

Document Type

Graduate Thesis - Open Access

Major

Geosciences

Degree Name

Master of Science

College

College of Arts and Sciences

Department

Department of Geosciences

Abstract

The Lowry Method of Protein Assay is an effective alternative to point count or culture methods to determine the relative abundance of microorganisms in geologic samples. Results of this project show that the outcome of the Lowry assay is not affected by the constituents of a sandstone reservoir and that a relationship exists between microbes and clay minerals. Core samples were taken from the Carter sandstone at the North Blowhorn Creek Unit in Lamar County, Alabama. Samples were chosen based on mineralogic heterogeneity. The samples were placed in an anaerobic glove bag and given nutrients to induce the growth of in situ microorganisms. Samples were then assayed. Results of the protein assays show that the Lowry Method of Protein Assay is effective in determining protein concentrations in geologic samples with varying mineralogies. The assays also indicated that samples with abundant clay minerals show the greatest amount of microbial growth.

URI

https://hdl.handle.net/11668/19253

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