Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Shan, Xueyan

Committee Member

Williams, W. P.

Committee Member

Li, Jiaxu

Date of Degree

4-30-2021

Original embargo terms

Complete embargo for 2 years

Document Type

Graduate Thesis - Open Access

Major

Agriculture and Life Sciences

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Abstract

Aflatoxin is a highly carcinogenic secondary metabolite produced by Aspergillus flavus contributing to significant loss of corn production. There are several breeding techniques to make the corn resistant to aflatoxin accumulation with the genotype having desirable characters. Corn- resistant inbred line Mp313E provides resistance against A. flavus. Near-isogenic lines containing QTLs were developed by backcrossing Mp313E (resistant parent) and Va35 (susceptible parent). WRKY transcription factors play an important role in the growth and development of plants. Searching DNA markers associated with these WRKY genes is a considerable approach for the plant breeders. In this study, PCR and agarose gel electrophoresis methods were used to determine the polymorphic WRKY gene DNA markers associated with aflatoxin reduction quantitative trait loci in maize. The analysis revealed 26 polymorphic maize WRKY genes. Computational 3D models of these WRKY genes were predicted. Virtual 2D plot was made for these WRKY proteins to determine their position.

Sponsorship

USDA

Available for download on Monday, May 15, 2023

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