Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Shan, Xueyan
Committee Member
Williams, W. P.
Committee Member
Li, Jiaxu
Date of Degree
4-30-2021
Original embargo terms
Complete embargo for 2 years
Document Type
Graduate Thesis - Open Access
Major
Agriculture and Life Sciences
Degree Name
Master of Science
College
College of Agriculture and Life Sciences
Department
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology
Abstract
Aflatoxin is a highly carcinogenic secondary metabolite produced by Aspergillus flavus contributing to significant loss of corn production. There are several breeding techniques to make the corn resistant to aflatoxin accumulation with the genotype having desirable characters. Corn- resistant inbred line Mp313E provides resistance against A. flavus. Near-isogenic lines containing QTLs were developed by backcrossing Mp313E (resistant parent) and Va35 (susceptible parent). WRKY transcription factors play an important role in the growth and development of plants. Searching DNA markers associated with these WRKY genes is a considerable approach for the plant breeders. In this study, PCR and agarose gel electrophoresis methods were used to determine the polymorphic WRKY gene DNA markers associated with aflatoxin reduction quantitative trait loci in maize. The analysis revealed 26 polymorphic maize WRKY genes. Computational 3D models of these WRKY genes were predicted. Virtual 2D plot was made for these WRKY proteins to determine their position.
Sponsorship
USDA
Recommended Citation
Awasthi, Akanksha, "Polymorphism of WRKY factors in corn inbred lines carrying aflatoxin resistance quantitative trait loci" (2021). Theses and Dissertations. 5077.
https://scholarsjunction.msstate.edu/td/5077