Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Thomason, John M.

Committee Member

Mackin, Andrew J.

Committee Member

Williams, Matthew

Committee Member

Natalini, Claudio Correa

Committee Member

Howell, George Eli III

Date of Degree

4-30-2021

Original embargo terms

Worldwide

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medical Research

Degree Name

Master of Science

College

College of Veterinary Medicine

Department

Department of Clinical Sciences

Abstract

In humans, washing stored blood products prior to transfusion reduces storage lesions and the potential for transfusion reaction, but the effectiveness of washing units of canine whole blood is unknown. The objective of this study was to determine if a manual method of washing of stored whole blood units reduced storage lesions without adversely affecting erythrocytes. Units of canine whole blood were stored for 28 days and manually washed three times with sterile .9% NaCl. Following the first wash, there was a decrease in serum potassium (P<.0001), lactate (P<.0001), pH (P=.0110), pCO2 (P<.0001), TCO2 (P<.0001), arachidonic acid (P<.0001), and thromboxane B2 (P=.0417), and increases in iCa (P=.0494), iMg (P=.0024), MCV (P<.0001), MCHC (P=.0093), RDW (P=.0009), hemoglobin (P=.0011), and MCF (P=.0006). No bacterial growth was identified on the post-transfusion samples. Manual washing of stored blood significantly reduces storage lesions after a single wash and additional washing may cause in vitro hemolysis.

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