Mississippi State University
Tomberlin, Jeffery K.
Date of Degree
Graduate Thesis - Open Access
Master of Science (M.S.)
College of Arts and Sciences
Department of Biological Sciences
With a growing global population, food security and waste management strategies are needed (FAO, 2002b); insects have been promoted for these goals. Black soldier fly larvae (BSFL) are endorsed for their ability in decomposition and use as feed (Bava et al., 2019; Swett Walker, 2018). Studies show the life cycle of BSFL is impacted by bacterial supplementation (Franks et al., 2021; Kooienga et al., 2020). We seek to determine the effects of supplementing BSFL diet with Pseudomonas putida 3P. We conducted two forms of a 10-day bench-top study observing larval mass, frass conversion, C:N content, and ammonia production with supplementation of Pseudomonas putida 3P in single and double-doses. Supplementation with P. putida results in roughly 1.5X larger larval wet and dry mass, variable concentrations of ammonia, and approximately 1.15X smaller C:N ratios in frass. This suggests interruption of larval rearing may affect volatile ammonia concentrations and inhibit P. putida behavior.
Waters, Rebecca, "Determining the effectfs of introducing Pseudomonas putida 3P to Black Soldier Fly (Hermetia illucens) Gainesville diet" (2022). Theses and Dissertations. 5517.