Theses and Dissertations

Issuing Body

Mississippi State University


Peebles, E. David

Committee Member

Evans, Jeff D.

Committee Member

Leigh, Spencer

Committee Member

Adhikari, Pratima

Date of Degree


Document Type

Graduate Thesis - Campus Access Only


Poultry Science

Degree Name

Master of Science (M.S.)


College of Agriculture and Life Sciences


Department of Poultry Science


Avian Pathogenic E. coli can lead to infections in laying hens and wide-spread economic problems in the table egg industry. A vaccine widely used to combat APEC is the Poulvac E. coli vaccine, which is normally applied via spray vaccination. Application of this vaccine via in ovo injection has not been examined. The purpose of this series of studies was to determine the effects of injecting 50 μL of different dosages of the PECV into Hy-Line W-36 layer hatching eggs on the hatchability and characteristics of the hatchlings, and on the livability and characteristics of the chicks through 21 d growout. Administration of the PECV at 18 d of incubation yielded unsatisfactory results in terms of hatchability and the characteristics of the chicks at hatch and through 21 d of age. Commercial use of the PECV via in ovo injection in Hy-Line W-36 layer hatching eggs is not recommended.


United States Department of Agriculture (USDA; agreement No. 58-6064-9-014)