Theses and Dissertations

Author

Yuqing Tan

Issuing Body

Mississippi State University

Advisor

Chang, Sam K.C.

Committee Member

Edelmann, Mariola J.

Committee Member

Sepehrifar, Mohammad

Committee Member

Cheng, Wen-Hsing

Committee Member

Haque, Zahur Z.

Date of Degree

1-1-2015

Document Type

Graduate Thesis - Open Access

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Abstract

Antioxidant-rich plant foods can inhibit starch and lipid digestion that are relevant to the management of type-II diabetes. Our objective was to compare the three phenolic substances (total phenolic, total flavonoids, and condensed tannin content) in crude, semi-purified extracts from eight types of foods (purified by XAD-7 column), five fractions (semi-purified extracts fractionated by Sephadex LH-20 column) from black legumes, and to compare their antioxidant capacities. The IC50 values of these crude extracts, semi-purified extracts and fractions against alpha-amylase, alpha-glucosidase and lipase were measured. Results showed that Fraction V from black soybean had the lowest IC50 value (0.25 mg/mL) against alpha-amylase; Fraction V from black bean had the lowest IC50 value (0.25 micro gram/mL) against alpha-glucosidase; Fraction IV of black bean had the lowest IC50 value (76 micro gram/mL) against lipase; myricetin showed the lowest IC50 value against alpha-amylase, alpha-glucosidase and lipase.

URI

https://hdl.handle.net/11668/19903

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