Theses and Dissertations

Ismail Khan

Issuing Body

Mississippi State University

Advisor

Nikolic, Dragica Jeremic

Committee Member

Hassan, El Barbary

Committee Member

Stokes, C. Elizabeth

Committee Member

Klink, Vincent

Date of Degree

5-6-2017

Document Type

Graduate Thesis - Open Access

Major

Sustainable Bioproducts

Degree Name

Master of Science

College

College of Forest Resources

Department

Department of Sustainable Bioproducts

Abstract

Soybean (Glycine max) yield is significantly affected by soybean cyst nematode (SCN), Heterodera glycines, and causes an annual loss of billions of US dollars. In this study, Glycine max xyloglucan endotransglycosylase/hydrolase gene (Gm-XTH52) was transformed into a nematode susceptible G. max [Williams 82/PI 518671] variety of soybean to test whether the protein expression has a role in resistance to H. glycines, and possible chemical changes the expression may cause in the plant composition. Expression level of the Gm-XTH52 gene was three times higher than in controls. Significant reduction in the number of SCN cysts suggested suppression of H. glycines parasitism upon transformation. While total sugar amounts did not significantly differ between the transformed and control plants, xyloglucan amounts of loosely bound sugars of genetically mosaic plants were significantly lower in comparison to controls. Control plants showed lower molecular weight sugars than the transformed plants not subjected to H. glycines infection.

URI

https://hdl.handle.net/11668/17569

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