Abstract

Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium able to utilize CO and H(2) to derive energy for fixation of CO(2). Thus, it is capable of growth using syngas, which is a mixture of varying amounts of CO and H(2) generated by organic waste gasification. O. carboxidovorans is capable also of heterotrophic growth in standard bacteriologic media. Here we characterize how the O. carboxidovorans proteome adapts to different lifestyles of chemolithoautotrophy and heterotrophy. Fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown with acetate or with syngas showed that the bacterium changes membrane fatty acid composition. Quantitative shotgun proteomic analysis of O. carboxidovorans grown in the presence of acetate and syngas showed production of proteins encoded on the megaplasmid for assimilating CO and H(2) as well as proteins encoded on the chromosome that might have contributed to fatty acid and acetate metabolism. We found that adaptation to chemolithoautotrophic growth involved adaptations in cell envelope, oxidative homeostasis, and metabolic pathways such as glyoxylate shunt and amino acid/cofactor biosynthetic enzymes.

Publisher

Public Library of Science

DOI

10.1371/journal.pone.0017111

Publication Date

2-28-2011

College

College of Agriculture and Life Sciences| College of Veterinary Medicine| James Worth Bagley College of Engineering

Department

Department of Biochemistry and Molecular Biology| Department of Chemical Engineering

Research Center

Life Sciences and Biotechnology Institute

Keywords

Bacterial, Bacterial Proteins, Bacterial Proteins: analysis, Bradyrhizobiaceae, Bradyrhizobiaceae: enzymology, Bradyrhizobiaceae: genetics, Bradyrhizobiaceae: growth & development, Bradyrhizobiaceae: metabolism, Chemoautotrophic Growth, Chemoautotrophic Growth: physiology, Cluster Analysis, Fatty Acids, Fatty Acids: analysis, Gene Regulatory Networks, Gene Regulatory Networks: physiology, Genes, Glyoxylates, Glyoxylates: metabolism, Heterotrophic Processes, Heterotrophic Processes: physiology, Membrane Lipids, Membrane Lipids: analysis, Metabolic Networks and Pathways, Metabolic Networks and Pathways: genetics, Oxidation-Reduction, Proteome, Proteome: analysis

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