Willard, Scott T.
Williams, W. Paul
Date of Degree
Graduate Thesis - Open Access
Agricultural Life Sciences
Master of Science
College of Agriculture and Life Sciences
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology
Aspergillus flavus is a pathogenic fungus causing maize ear rot disease and producing aflatoxins that are carcinogenic to humans and animals. Characterizing maize host resistance mechanisms and prioritizing candidate resistance genes are important to the development of resistant maize germplasm. This study investigated transcriptomics approaches and statistics methods on the identification of maize host resistance genes. Full-length cDNA libraries were also constructed and evaluated for gene cloning and functional analysis. This study established important sources for functional studies on differentially expressed genes and for identification of allelic gene forms to characterize gene polymorphisms and facilitate the development of DNA markers.
Asters, Matthew Constantine, "Characterization of Maize Genes in Response to Aspergillus Flavus Infection and Aflatoxin Accumulation" (2014). Theses and Dissertations MSU. 1022.