Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Prewitt, Lynn M.

Committee Member

Ma, Din-Pow.

Committee Member

Jeremic, Dragica.

Committee Member

Borazjani, Hamid.

Committee Member

Willeford, Kenneth O.

Date of Degree

5-1-2013

Document Type

Graduate Thesis - Open Access

Major

Forest Products

Degree Name

Master of Science

College

College of Forest Resources

Department

Department of Forest Products

Abstract

The objective was to compare pentachlorophenol (PCP) degradation in contaminated groundwater by indigenous and bio-augmented (Sphingobium chlorophenolicum and Burkholderia cepacia) PCP degrading bacteria. Indigenous bacteria were identified by cloning and sequencing of 16S rDNA fragments while PCP concentrations were determined by GC-ECD. Gene expression for PCP degrading enzymes: chlorophenol 4-monooxygenase (TftD, B. cepacia) and pentachlorophenol-4-monooxygenase (pcpB, S. chlorophenolicum), was determined by RT-PCR. B. cepacia, a PCP degrading bacteria was identified as dominant indigenous bacteria. PCP concentrations correlated negatively with PCP tolerant bacteria and relative fold gene expression in treatments with air-sparging (phase2) compared to without air-sparging (phase1). PCP concentrations decreased and TftD or pcpB expressions were higher in treatments inoculated with B. cepacia (49%, 10.7 fold) or S. chlorophenolicum (32%, 7 fold), respectively, than un-inoculated (indigenous) or mixed culture inoculated treatments. Thus bio-augmentation of indigenous bacteria with B. cepacia or S. chlorophenolicum resulted in more PCP degradation than indigenous bacteria.

URI

https://hdl.handle.net/11668/16563

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