Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Prewitt, Lynn M.
Committee Member
Ma, Din-Pow.
Committee Member
Jeremic, Dragica.
Committee Member
Borazjani, Hamid.
Committee Member
Willeford, Kenneth O.
Date of Degree
5-11-2013
Document Type
Graduate Thesis - Open Access
Major
Forest Products
Degree Name
Master of Science
College
College of Forest Resources
Department
Department of Forest Products
Abstract
The objective was to compare pentachlorophenol (PCP) degradation in contaminated groundwater by indigenous and bio-augmented (Sphingobium chlorophenolicum and Burkholderia cepacia) PCP degrading bacteria. Indigenous bacteria were identified by cloning and sequencing of 16S rDNA fragments while PCP concentrations were determined by GC-ECD. Gene expression for PCP degrading enzymes: chlorophenol 4-monooxygenase (TftD, B. cepacia) and pentachlorophenol-4-monooxygenase (pcpB, S. chlorophenolicum), was determined by RT-PCR. B. cepacia, a PCP degrading bacteria was identified as dominant indigenous bacteria. PCP concentrations correlated negatively with PCP tolerant bacteria and relative fold gene expression in treatments with air-sparging (phase2) compared to without air-sparging (phase1). PCP concentrations decreased and TftD or pcpB expressions were higher in treatments inoculated with B. cepacia (49%, 10.7 fold) or S. chlorophenolicum (32%, 7 fold), respectively, than un-inoculated (indigenous) or mixed culture inoculated treatments. Thus bio-augmentation of indigenous bacteria with B. cepacia or S. chlorophenolicum resulted in more PCP degradation than indigenous bacteria.
URI
https://hdl.handle.net/11668/16563
Recommended Citation
Joshi, Vaibhav V., "Comparison of Indigenous and Bio-Augmented Pentachlorophenol (PCP) Degrading Bacteria for Remediation of PCP in Contaminated Groundwater" (2013). Theses and Dissertations. 1111.
https://scholarsjunction.msstate.edu/td/1111