Theses and Dissertations

Author

Dinum Perera

Issuing Body

Mississippi State University

Advisor

Trader, W. Brian

Committee Member

Peterson, Daniel

Committee Member

Caprio, A. Michael

Committee Member

Harkess, L. Richard

Date of Degree

8-1-2009

Document Type

Graduate Thesis - Open Access

Abstract

The broad objective of this research was to develop transgenic poinsettia that express tryptophan decarboxylase (TDC) capable of protecting poinsettia against whitefly. An effective and efficient in vitro micro propagation and proliferation technique of poinsettia ‘Prestige Red’ was successfully developed in this study and this protocol can be used for potential development of transgenic poinsettia. Poinsettia ‘Prestige Red’ was successfully infected by Agrobacterium rhizogenes producing hairy roots at the site of infection. Investigations of more effective PGR concentrations are necessary in order to develop transgenic poinsettia through hairy roots. Stem disks of poinsettia ‘Eckespoint Pollys Pink’ developed into somatic embryos when they were transformed by A. tumefaciens harboring TDC. A. tumefaciens-mediated transformation of poinsettia through somatic embryogenesis is cultivar dependent. Additional research into more effective PGR combinations, antibiotic concentrations and antinecrosis chemicals is required in order to develop transgenic poinsettia harboring TDC through somatic embryogenesis using A. tumefaciens.

URI

https://hdl.handle.net/11668/15438

Share

COinS