Theses and Dissertations


Amanda Gaudin

Issuing Body

Mississippi State University


Wallace, Ted P.

Committee Member

Scheffler, Jodi

Committee Member

Stetina, Sally

Committee Member

Henry, W. Brien

Committee Member

Wubben, Martin

Date of Degree


Document Type

Graduate Thesis - Open Access



Degree Name

Master of Science (M.S.)


College of Agriculture and Life Sciences


Department of Plant and Soil Sciences


Two sources of reniform nematode (Rotylenchulus reniformis) resistance in two Upland cotton (Gossypium hirsutum) developed from related cotton species, BARBREN-713 (Renbarb1 and Renbarb2 genes), and the LONREN germplasm lines (Renlon gene) are available to breeders. Stunting was observed in the LONREN source of resistance, but not for BARBREN. To investigate future use of LONREN germplasm, lines were developed with different combinations of genes from both the LONREN and BARBREN-713 germplasm lines. Marker assisted selection (MAS) was conducted identify these gene combinations within breeding population 1) Renbarb1 +Renbabr2 2) Renbarb1 + Renbar2 + Renlon 3) Renbarb2 + Renlon 4) Renbarb2 5) Renbarb1 6) Renbarb1+Renlon 7) Renlon, and 8) a susceptible genotype. Two populations were identified and increased in 2015. Genotypes were inoculated with 0, 2300, or 5000 reniform nematodes. Seedlings were harvested at 90 days to collect plant measurements and RF of reniform nematodes from roots and soil. The Renbarb1 genotype was susceptible. No significant differences in genotypes were observed for root and shoot measurements, and there was no indication of stunting.