Advisor

Mackin, Andrew

Committee Member

Pinchuk, Lesya

Committee Member

Langston, Cory

Committee Member

Pruett, Stephen

Date of Degree

1-1-2012

Document Type

Graduate Thesis - Open Access

Abstract

Cyclosporine is a potent immunosuppressive agent used to treat a wide range of canine inflammatory diseases. Unfortunately, optimal dosing protocols for achieving immunosuppression with cyclosporine in dogs remain unclear, and standard methods that objectively monitor effectiveness of immunosuppression have not been established. We evaluated an already established panel of biomarkers of immunosuppression in vivo with two oral dosages of cyclosporine in seven normal dogs, a high dosage known to induce immunosuppression and a lower dosage used to treat atopy, with a washout period between the two dosages. The biomarker panel included the flow cytometric evaluation of T-lymphocyte cytokine expression (IL-2, IL-4, and IFN-gamma). High dosage cyclosporine resulted in significant decreases in IL-2 and INF-gamma expression, but not IL-4 expression. Low dosage cyclosporine was associated with a significant decrease in INF-gamma expression, while IL-2 expression was not affected. The results demonstrated suppression of biomarkers in a dose-dependent manner.

URI

https://hdl.handle.net/11668/20546

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