Hydrolysis of Organophosphate and Model Substrates in African American and Caucasian Southerners by Serum Paraoxonase-1 (pon1) and its Relationship to Atherosclerosis

Author

Ryan Hunter Coombes

Issuing Body

Mississippi State University

Advisor

Chambers, Janice E.

Committee Member

Crow, John A.

Committee Member

Wills, Robert W.

Date of Degree

12-9-2011

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medical Science

Degree Name

Master of Science (M.S.)

College

College of Veterinary Medicine

Department

Veterinary Medical Science Program

Abstract

Paraoxonase-1 (PON1) is a high density lipoprotein (HDL)-associated enzyme displaying esterase and lactonase activity. PON1 hydrolyzes the oxons of several organophosphorous insecticides (e.g. paraoxon, diazoxon and chlorpyrifos-oxon) and metabolizes lipid peroxides of low density lipoproteins (LDL) and HDL. As such, PON1 plays a relevant role in determining susceptibility of organophosphate toxicity and cardiovascular disease. The objective of this study was to determine associations of PON1 status (i.e. genotype and activity levels) with atherosclerosis (ATH) in individuals from the Southeastern United States. An additional objective was to determine whether PON1 genotype and/or PON1 activity levels influence the capacity of PON1 to metabolize chlorpyrifos-oxon (CPO) at a relatively low concentration. Data indicated increasing PON1 activity assessed by hydrolysis of phenyl acetate is associated with decreased odds of ATH. Furthermore, neither PON1 genotype nor PON1 activity levels influence capacity of PON1 to metabolize CPO at a relatively low concentration.

URI

https://hdl.handle.net/11668/20399

Recommended Citation

Coombes, Ryan Hunter, "Hydrolysis of Organophosphate and Model Substrates in African American and Caucasian Southerners by Serum Paraoxonase-1 (pon1) and its Relationship to Atherosclerosis" (2011). Theses and Dissertations. 2531.
https://scholarsjunction.msstate.edu/td/2531