Theses and Dissertations

Guohua Yang

Issuing Body

Mississippi State University

Advisor

Wan, Xiu-Feng (Henry)

Committee Member

Hanson, Larry

Committee Member

Cooley, Avery James

Committee Member

Ross, Matthew K.

Date of Degree

12-15-2012

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medical Science

Degree Name

Master of Science

College

College of Veterinary Medicine

Department

Veterinary Medical Science Program

Abstract

Emergence of avian origin and equine origin canine influenza viruses (CIVs) in Asia and the United States brings important concerns. Humans are in closer and more frequent contact with dogs than other common hosts of influenza. Thus, CIV is a potential threat to human health. However, little is known about the determinants of CIV host tropism or the transmissibility of CIVs to humans. An amino acid change (W222L) was implicated in modifying hemagglutinin receptor binding by CIV. This was tested using reverse genetics, glycan microarray and virus histochemistry. Glycan microarray demonstrated that avian-origin CIV (H3N2-222W) bind predominantly to alpha-2, 3 linked glycans. Virus histochemistry indicated that rH3N2-222L had higher binding affinity with epithelial cilia of canine tracheal tissue and weaker binding with avian tracheal tissue. Ferret infection demonstrated that the avian-origin H3N2 CIV could cause infection and limited to rhinitis, suggesting that CIV could infect humans.

URI

https://hdl.handle.net/11668/19074

Comments

receptor binding affinity||canine influenza A virus||infectivity||glycan microarray

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