Mississippi State University
Brown, Richard L.
Outlaw, Diana C.
Baker, Gerald T.
Date of Degree
Graduate Thesis - Open Access
Master of Science
College of Agriculture and Life Sciences
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology
A comprehensive review of whole mount staining revealed an enhanced staining method to improve visibility of morphological structures. Ultimately, five factors vital to stain quality were identified, with pH being a primary factor. Phylogenetic relationships of all 22 tribes of Tortricidae, representing 57 genera and 78 species (distributed in Neotropical, Nearctic, Palearctic, Oriental, and Australian regions), were investigated based on 52 morphological characters, including incorporation of 27 novel non-traditional characters of the endo- and exoskeleton. The phylogenetic analysis yielded five equally parsimonious trees (length 389 steps, CI=0.2571, RI=0.7051), a strict consensus (length 392 steps, CI=0.2551, RI= 0.7021) of which produced two trichotomies. These results reinforced those from previous molecular analyses (Fagua et al., 2016; Regier et al., 2016) with some disagreement, consistent with historical conclusions made based on morphology. Mapping character distribution onto a recent molecular based phylogeny (Regier et al., 2012) revealed promising characters for subfamily and tribal delimitation
Jaeger, Christi, "Phylogeny of Tortricidae (Lepidoptera) a Morphological Approach with Enhanced Whole Mount Staining Techniques" (2017). Theses and Dissertations. 3531.