Author

Sana Mujahid

Advisor

Wang, Chinling

Committee Member

Boyle, John

Committee Member

Ma, Peter

Date of Degree

1-1-2007

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medical Science

Degree Name

Master of Science

College

College of Veterinary Medicine

Abstract

Listeria monocytogenes is a deadly, Gram-positive foodborne pathogen that is ubiquitous in the environment. The bacterium expresses a number of virulence and stress adaptation proteins that support its pathogenic capabilities. Two-dimensional gel electrophoresis (2-DE) was used to map L. monocytogenes surface proteins, which play a central role in virulence, and to examine protein expression by L. monocytogenes grown on ready-to-eat meat, an important source of Listeria infections. A novel method for solubilization of surface proteins from L. monocytogenes for 2-DE was developed. Additionally, the unique proteome expressed by L. monocytogenes grown on a meat matrix was uncovered. The developed solubilization method will facilitate efforts to identify and routinely compare surface proteins of Listeria by 2-DE. Furthermore, the 2-DE database of proteins expressed by L. monocytogenes grown on a meat matrix will allow further understanding of the interactions of Listeria with its food environment that influence its ability to cause disease.

URI

https://hdl.handle.net/11668/17017

Comments

two-dimensional gel electrophoresis||proteomics||Listeria monocytogenes

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