Title

Proteomic analysis of outer membrane vesicles of Aeromonas hydrophila ML09-119

Advisor

Lawrence, Mark L.

Committee Member

Karsi, Attila

Committee Member

Hanson, Larry

Date of Degree

12-1-2020

Original embargo terms

Visible to MSU only for 1 Year||12/15/2021

Document Type

Graduate Thesis - Open Access

Major

Veterinary Medical Science

Degree Name

Master of Science

College

College of Veterinary Medicine

Department

Department of Comparative Biomedical Sciences

Abstract

Aeromonas hydrophila ML09-119 is an important fish pathogen that severely affects channel catfish aquaculture. To better understand this strain’s virulence factors, outer membrane vesicles (OMVs) were isolated, and their proteome was assessed. Using transmission electron microscopy and dynamic light scattering, OMVs were shown to be monodispersed particles with an average diameter of 120.33 nm. OMV proteins were identified using mass spectrometry, and analysis of the resulting proteome of 74 proteins revealed that many originated from the cytoplasm, but there was an enrichment of outer membrane, periplasmic, and extracellular proteins compared to the total proteome. The majority of the functional classifications were associated with bacterial metabolism. Of the predicted virulence factors, several had a putative function in adherence, and there were type III secretions system proteins as well as three secreted exotoxins. Overall, our data reveal new insights into A. hydrophila OMVs and their potential roles in physiology and virulence.

URI

https://hdl.handle.net/11668/20878

Comments

outer membrane vesicles||aeromonas hydrophila

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