Title

Purification and characterization of CopR, a copper(II) dependent transcription factor

Advisor

Emerson, Joseph P.

Committee Member

Fitzkee, Nicholas C.

Committee Member

Mlsna, Debra Ann

Committee Member

Travis

Date of Degree

8-1-2019

Original embargo terms

7/17/2022||Visible to MSU only for 3 years||

Document Type

Graduate Thesis - Open Access

Major

Chemistry

Degree Name

Master of Science

College

College of Arts and Sciences

Department

Department of Chemistry

Abstract

Transcription factors (TFs) are proteins that respond to a specific chemical signal and bind to DNA. In some bacteria, TFs control transition metal ion homeostasis by specifically binding with a particular metal ion or ions and then interacting with DNA. Although most first row metal ions are required as micronutrients for life, many of them can cause cellular damage or death if their concentrations are too high; this makes these TFs and their biological interactions excellent targets for drug development. The bacteria, Streptococcus pneumoniae is a pathogenic microorganism responsible for a range of diseases that target the young and the old, including pneumonia, meningitis, and bacteremia. Herein, we describe our efforts to study the Streptococcus pneumoniae TF responsible for copper(II) ion homeostasis. This thesis describes the classical biochemical techniques used to over-express and purify CopR. It also describes a series of preliminary characterization data associated with this novel copper(II)-dependent TF.

URI

https://hdl.handle.net/11668/14529

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