Theses and Dissertations

Issuing Body

Mississippi State University


Simpson, Chartrisa LaShan

Committee Member

Liao, Jun

Committee Member

Howell, George Eli, III

Committee Member

Elder, Steven H.

Date of Degree


Document Type

Graduate Thesis - Open Access


Biomedical Engineering

Degree Name

Master of Science


James Worth Bagley College of Engineering


Department of Agricultural and Biological Engineering


Cardiovascular disease is most deadly medical condition in the United States. Medial vascular calcification is a disease that often precedes other more serious cardiovascular diseases that have high mortality. In order to research new therapies for the treatment of medial vascular calcification, an in vitro cell culture model must be developed that mimics the process in vivo. This disease is shown to be an active, cell-mediated process where the vascular smooth muscle cells (VSMCs) in the arteries are differentiating into osteoblast-like cells and depositing hydroxyapatite mineral in the artery walls. By administering inorganic phosphate to cell culture medium, an osteogenic shift can initiated in VSMCs in vitro resulting in calcium deposition and an increase in bone related proteins. We propose to develop and characterize a model for vascular calcification and investigate the effects of magnesium supplementation on in vitro calcification and cellular phosphate uptake.



Chronic Kidney Disease||Osteogenic Differentiation