Advisor

Simpson, Chartrisa LaShan

Committee Member

Liao, Jun

Committee Member

Howell, George Eli, III

Committee Member

Elder, Steven H.

Date of Degree

1-1-2015

Document Type

Graduate Thesis - Open Access

Major

Biomedical Engineering

Degree Name

Master of Science

College

James Worth Bagley College of Engineering

Department

Department of Agricultural and Biological Engineering

Abstract

Cardiovascular disease is most deadly medical condition in the United States. Medial vascular calcification is a disease that often precedes other more serious cardiovascular diseases that have high mortality. In order to research new therapies for the treatment of medial vascular calcification, an in vitro cell culture model must be developed that mimics the process in vivo. This disease is shown to be an active, cell-mediated process where the vascular smooth muscle cells (VSMCs) in the arteries are differentiating into osteoblast-like cells and depositing hydroxyapatite mineral in the artery walls. By administering inorganic phosphate to cell culture medium, an osteogenic shift can initiated in VSMCs in vitro resulting in calcium deposition and an increase in bone related proteins. We propose to develop and characterize a model for vascular calcification and investigate the effects of magnesium supplementation on in vitro calcification and cellular phosphate uptake.

URI

https://hdl.handle.net/11668/18965

Comments

Chronic Kidney Disease||Osteogenic Differentiation

Share

COinS