Date of Degree
Graduate Thesis - Open Access
Master of Science
College of Engineering
Department of Agricultural and Biological Engineering
Articular cartilage provides a near frictionless surface for the articulating ends of bones. Cartilage functions to lubricate and transmit compressive forces resulting from joint loading and impact. If damaged, whether by traumatic injury or disease, cartilage lacks the ability for self-repair. This study explores the production of scaffoldree cartilage and investigates the effect of Tissue Growth Technologies’ CartiGen Bioreactor on the cartilage. Chondrocyte and bone marrow-derived stem cell (BMSC) attachment to chitosan is also investigated in hopes of producing a bilayered construct for osteochondral repair. Results demonstrate that culturing of scaffoldree cartilage in the CartiGen bioreactor resulted in an enhancement of the scaffoldree cartilage’s biomechanical and biochemical properties and that the chitosan microspheres were able to successfully support porcine chondrocyte and BMSC attachment. Results from both studies are encouraging for future work involving tissue engineered cartilage.
Tran, Scott Chi, "Tissue engineering cartilage for focal defects" (2010). Theses and Dissertations MSU. 4738.