Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Aarattuthodi, Suja

Committee Member

Phelps, Nicholas B. D.

Committee Member

Khoo, Lester H.

Committee Member

Karunakaran, Ganesh K.

Date of Degree

4-30-2021

Original embargo terms

Complete embargo for 2 years

Document Type

Graduate Thesis - Open Access

Major

Wildlife, Fisheries and Aquaculture

Degree Name

Master of Science (M.S.)

College

College of Forest Resources

Department

Department of Wildlife, Fisheries and Aquaculture

Abstract

Lack of host-specific cell cultures necessitated initiation of primary cell cultures from ictalurid catfish. Cell cultures from the fin tissues of hybrid catfish ( channel catfish x blue catfish) were developed, characterized, and species of origin molecularly authenticated. Blue catfish alloherpesvirus (BCAHV) is an Ictalurid herpesvirus. When BCAHV was inoculated onto various fish cell lines from different families, the cytopathic effects were restricted to cell lines from family Ictaluridae indicating the host-specificity of virus. A virus challenge using channel, blue, and hybrid catfish indicated mortality due to BCAHV was significantly higher in blue and hybrid catfish. Crowding influenced BCAHV pathogenesis indicated by significantly higher mortality in highly stocked tanks. Host susceptibility to BCAHV differed with age. Temperature had a significant role in the activation and pathogenesis of BCAHV. The enhanced virulence of BCAHV in blue and hybrid catfish reveals its potential to be a significant pathogen in catfish culture.

Sponsorship

Mississippi Agricultural and Forestry Experiment Station - Strategic Research Initiative (MAFES-SRI)

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