Application of Raman and fluorescence spectroscopy to detect changes in the chemical profile of water subject to polarization, vegetation under stress, and murine blood components

Author

Supriya Nagpal

Issuing Body

Mississippi State University

Advisor

Ariunbold, Gombojav O.

Committee Member

Pradhan, Prabhakar

Committee Member

Arnoldus, Hendrik F.

Date of Degree

8-9-2019

Original embargo terms

Worldwide

Document Type

Graduate Thesis - Open Access

Major

Physics

Degree Name

Master of Science

College

College of Arts and Sciences

Department

Department of Physics and Astronomy

Abstract

This thesis broadly describes the construction of two kinds of spectroscopic set-ups to analyze properties of various materials. In the first part, construction of a Raman spectrometer and a high-throughput in-vivo detection for early plant abiotic stress responses is described. Following which, the set-up is modified into a microscope employed to study Murine blood components with samples varying in age. Initial Raman set-up is also improvised using a polarizer in order to gain deeper understanding of the vibrational and rotational bonds in water. The second part of the thesis explains the construction of a laser-induced fluorescence (LIF) sensor module. Performance testing and experiments were carried out with the sensor module to monitor stress in vegetation and fruits and also detect toxins found in corn and carcinogenic compounds in gasoline. The module was further mounted to an unmanned aerial vehicle for field surveys and preliminary testing in flight is described.

URI

https://hdl.handle.net/11668/14599

Recommended Citation

Nagpal, Supriya, "Application of Raman and fluorescence spectroscopy to detect changes in the chemical profile of water subject to polarization, vegetation under stress, and murine blood components" (2019). Theses and Dissertations. 713.
https://scholarsjunction.msstate.edu/td/713