A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.
Public Library of Science
College of Agriculture and Life Sciences| College of Arts and Sciences
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology| Department of Biological Sciences
Animals, Base Sequence, Caenorhabditis elegans Proteins, Caenorhabditis elegans Proteins: genetics, DNA, DNA Primers, DNA Primers: genetics, Metagenome, Metagenome: genetics, Microfilament Proteins, Microfilament Proteins: genetics, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Analysis, Soil, Soil: chemistry, Soil: parasitology, Species Specificity, Tylenchoidea, Tylenchoidea: genetics
Li, Yan; Lawrence, Gary W.; Lu, Shien; Balbalian, Clarissa; and Klink, Vincent P., "Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production." (2014). College of Agriculture & Life Sciences Publications and Scholarship. 6.