Abstract
A quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.
Publisher
Public Library of Science
Publication Date
12-16-2011
College
College of Agriculture and Life Sciences| College of Arts and Sciences
Department
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology| Department of Biological Sciences
Keywords
Animals, Base Sequence, DNA, DNA Primers, DNA Primers: metabolism, Geographic Information Systems, Helminth, Helminth: analysis, Helminth: genetics, Helminth: isolation & purification, Metagenomics, Metagenomics: methods, Molecular Sequence Data, Polymerase Chain Reaction, Reference Standards, Soil, Soil: parasitology, Tylenchoidea, Tylenchoidea: genetics
Disciplines
Soil Science
Recommended Citation
Showmaker K, Lawrence GW, Lu S, Balbalian C, Klink VP (2011) Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil. PLoS ONE 6(12): e28954. https://doi.org/10.1371/journal.pone.0028954
