Title

Quantitative field testing Rotylenchulus reniformis DNA from metagenomic samples isolated directly from soil.

Abstract

A quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.

Publisher

Public Library of Science

DOI

10.1371/journal.pone.0028954

Publication Date

12-16-2011

College

College of Agriculture and Life Sciences| College of Arts and Sciences

Department

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology| Department of Biological Sciences

Keywords

Animals, Base Sequence, DNA, DNA Primers, DNA Primers: metabolism, Geographic Information Systems, Helminth, Helminth: analysis, Helminth: genetics, Helminth: isolation & purification, Metagenomics, Metagenomics: methods, Molecular Sequence Data, Polymerase Chain Reaction, Reference Standards, Soil, Soil: parasitology, Tylenchoidea, Tylenchoidea: genetics

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