A quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil-dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil.
Public Library of Science
College of Agriculture and Life Sciences| College of Arts and Sciences
Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology| Department of Biological Sciences
Animals, Base Sequence, DNA, DNA Primers, DNA Primers: metabolism, Geographic Information Systems, Helminth, Helminth: analysis, Helminth: genetics, Helminth: isolation & purification, Metagenomics, Metagenomics: methods, Molecular Sequence Data, Polymerase Chain Reaction, Reference Standards, Soil, Soil: parasitology, Tylenchoidea, Tylenchoidea: genetics
Showmaker K, Lawrence GW, Lu S, Balbalian C, Klink VP (2011) Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil. PLoS ONE 6(12): e28954. https://doi.org/10.1371/journal.pone.0028954