Global analysis of lysine acetylation suggests the involvement of protein acetylation in diverse biological processes in rice (Oryza sativa).

Authors

Babi Ramesh Reddy Nallamilli
Mariola J. Edelmann
Xiaoxian Zhong
Feng Tan
Hana Mujahid
Jian Zhang
Bindu Nanduri, Mississippi State UniversityFollow
Zhaohua Peng, Mississippi State UniversityFollow

ORCID

Bindu Nanduri: https://orcid.org/0000-0002-9996-2976

Abstract

Lysine acetylation is a reversible, dynamic protein modification regulated by lysine acetyltransferases and deacetylases. Recent advances in high-throughput proteomics have greatly contributed to the success of global analysis of lysine acetylation. A large number of proteins of diverse biological functions have been shown to be acetylated in several reports in human cells, E.coli, and dicot plants. However, the extent of lysine acetylation in non-histone proteins remains largely unknown in monocots, particularly in the cereal crops. Here we report the mass spectrometric examination of lysine acetylation in rice (Oryza sativa). We identified 60 lysine acetylated sites on 44 proteins of diverse biological functions. Immunoblot studies further validated the presence of a large number of acetylated non-histone proteins. Examination of the amino acid composition revealed substantial amino acid bias around the acetylation sites and the amino acid preference is conserved among different organisms. Gene ontology analysis demonstrates that lysine acetylation occurs in diverse cytoplasmic, chloroplast and mitochondrial proteins in addition to the histone modifications. Our results suggest that lysine acetylation might constitute a regulatory mechanism for many proteins, including both histones and non-histone proteins of diverse biological functions.

Publisher

Public Library of Science

DOI

https://doi.org/10.1371/journal.pone.0089283

Publication Date

2-20-2014

College

College of Agriculture and Life Sciences

Department

Department of Biochemistry and Molecular Biology

Keywords

Acetylation, Affinity, Amino Acid Sequence, Biological Processes, Blotting, Chromatography, Gene Ontology, Humans, Lysine, Lysine: chemistry, Lysine: metabolism, Molecular Sequence Data, Oryza sativa, Plant Proteins, Plant Proteins: genetics, Plant Proteins: metabolism, Post-Translational, Protein Processing, Proteome, Proteome: analysis, Proteomics, Tandem Mass Spectrometry, Western

Disciplines

Biochemistry

Recommended Citation

Nallamilli BRR, Edelmann MJ, Zhong X, Tan F, Mujahid H, Zhang J, et al. (2014) Global Analysis of Lysine Acetylation Suggests the Involvement of Protein Acetylation in Diverse Biological Processes in Rice (Oryza sativa). PLoS ONE 9(2): e89283. https://doi.org/10.1371/journal.pone.0089283